NCT03190421
Expanded Quantitative Urinary Culture (EQUC) vs Standard Culture (SUC) Techniques in the Clinical Care
NA
COMPLETED
NCT03190421
INTERVENTIONAL
The Use of Expanded Quantitative Urinary Culture (EQUC) Versus Standard Culture (SUC) Techniques in the Clinical Care of Women With Symptoms of Urinary Tract Infections.
This purpose of this study is to see if expanded urine culture techniques used in the laboratory improve the clinical care of women over standard urine culture techniques.
Inclusion Criteria:
* A "yes" answer to the screening question "do you feel you have a UTI"?
* Non-pregnant women ages 18 years or older
* Agreement to respond to a text or email question 7-10 days after treatment plan for their UTI (note: the treatment plan may include "no treatment").
Exclusion Criteria:
* Women currently on antibiotics
* Patients who cannot communicate or read in English
* Patients under the age of 18
* Pregnant patients
* Women with an indwelling catheter and intermittent self-catheterization
* Men
* Urine obtained via the "clean catch method" (i.e. voided urine)
* Women who refuse to be catheterized
* Women who cannot or will not agree to respond to an email or text message 7-10 days after treatment plan is initiated.
Urinary Tract Infections
- DIAGNOSTIC
-
- Type: DIAGNOSTIC_TEST
- Name: Expanded Urinary Culture
- Description: involves the inoculation of 100X (0.1mL) more urine onto diverse types of media (BAP, chocolate agar, colistin and nalidixic acid (CNA) agar, CDC anaerobe 5% BAP) with incubation in more environments and temperatures (5% CO2 at 35°C for 48 h, aerobic conditions at 35°C and 30°C for 48 h, Campy gas mixture (5% O2, 10% CO2, 85% N) or anaerobic conditions at 35°C for 48 h). The level of detection for EQUC is 10 CFU/mL, represented by 1 colony of growth on any of the plates. EQUC is designed to isolate a broad array of Gram-negative and Gram-positive bacteria, including anaerobes and fastidious bacteria that grow slowly.
- Arm Group Labels: Expanded Urinary Culture (EQUC)
-
- Type: DIAGNOSTIC_TEST
- Name: Standard Urine Culture (SUC)
- Description: involves inoculation of 0.001 mL of urine onto 5% sheep blood agar plate (BAP) and MacConkey agar plate with the plates being incubated aerobically at 35°C for 24 h. Thus, the level of detection for standard culture is 103 CFU/mL, represented by 1 colony of growth on either plate. Standard culture is designed specifically to grow Gram-negative rods, especially UPEC.
- Arm Group Labels: Standard Urine Culture (SUC)
- Loyola University